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human serum complement quidel cat  (Quidel)


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    Quidel human serum complement quidel cat
    Human Serum Complement Quidel Cat, supplied by Quidel, used in various techniques. Bioz Stars score: 95/100, based on 233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+complement+serum/Normal+Human+Serum+-+Complement/us12570746-557-1-4
    Average 95 stars, based on 233 article reviews
    human serum complement quidel cat - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Selective depletion of tumor-infiltrating regulatory T cells with BAY 3375968, a novel Fc-optimized anti-CCR8 antibody
    Article Snippet: .. These cells were then exposed to CDC-qualified human complement serum (Quidel) and various antibodies in a dose-response manner at 37 °C for 3 h. Cells were stained with 7-AAD and cell death induced by CDC is indicated as percentage of 7-AAD-positive events, referencing to negative controls wells with either Treg cells only or Treg cells with 10% serum. ..

    Suspension:

    Article Title: Biological Characterization of a Stable Effector Functionless (SEFL) Monoclonal Antibody Scaffold in Vitro
    Article Snippet: .. 25 μl of human complement serum (QUIDEL, San Diego, CA) was added to the cell suspension in each well and the suspension was incubated overnight at 37 °C/5% CO 2 . ..

    Incubation:

    Article Title: Biological Characterization of a Stable Effector Functionless (SEFL) Monoclonal Antibody Scaffold in Vitro
    Article Snippet: .. 25 μl of human complement serum (QUIDEL, San Diego, CA) was added to the cell suspension in each well and the suspension was incubated overnight at 37 °C/5% CO 2 . ..

    Article Title: Novel anti-4-1BB×PD-L1 bispecific antibody augments anti-tumor immunity through tumor-directed T-cell activation and checkpoint blockade
    Article Snippet: .. Serially diluted antibodies were then added to the wells and 25 μL of twofold diluted human complement serum (Quidel) was added to the well followed by incubation for 5 hours at 37°C. ..

    Article Title: LAG-3xPD-L1 bispecific antibody potentiates antitumor responses of T cells through dendritic cell activation
    Article Snippet: .. Serially diluted antibodies were added, and 25 μL of 2-fold diluted human complement serum (Quidel) was added to the well, followed by incubation for 5 h at 37 °C in 5% CO2 incubator. .. Next, 75 μL of CytoTox-Glo reagent (Promega) was added to each well, and luminescence was measured using a plate reader (PHERAstar).

    Article Title: LAG-3xPD-L1 bispecific antibody potentiates antitumor responses of T cells through dendritic cell activation
    Article Snippet: .. Serially diluted antibodies were added, and 25 μL of 2-fold diluted human complement serum (Quidel) was added to the well, followed by incubation for 5 h at 37 °C in 5% CO2 incubator. .. Next, 75 μL of CytoTox-Glo reagent (Promega) was added to each well, and luminescence was measured using a plate reader (PHERAstar).

    Article Title: T0001, a variant of TNFR2-Fc fusion protein, exhibits improved Fc effector functions through increased binding to membrane-bound TNFα
    Article Snippet: .. The cells and TNFα antagonists were then incubated in the presence of human complement serum (Quidel) to a final concentration of 8%, for 2 h at 37°C, 5% CO 2 . .. At the end of incubation, 50 μl of AlamarBlue TM (Invitrogen) was added to each well, and the plate was incubated for an additional 17 h under the same conditions.

    Concentration Assay:

    Article Title: T0001, a variant of TNFR2-Fc fusion protein, exhibits improved Fc effector functions through increased binding to membrane-bound TNFα
    Article Snippet: .. The cells and TNFα antagonists were then incubated in the presence of human complement serum (Quidel) to a final concentration of 8%, for 2 h at 37°C, 5% CO 2 . .. At the end of incubation, 50 μl of AlamarBlue TM (Invitrogen) was added to each well, and the plate was incubated for an additional 17 h under the same conditions.



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    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human <t>complement.</t> Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.
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    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human <t>complement.</t> Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.
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    Image Search Results


    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human complement. Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.

    Journal: Science Advances

    Article Title: Xenopus IgX informs engineering strategies of IgM and IgG hexamers

    doi: 10.1126/sciadv.aea3737

    Figure Lengend Snippet: ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human complement. Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.

    Article Snippet: The RTX-Fcμ or RTX-Fcγ proteins were incubated with equal volumes of cell cultures (~20,000 cells) and 12% normal human serum complement (Quidel) sequentially and then transferred into a 96-microwell plate.

    Techniques: Cell Culture